Recombinant human RCN3 protein
Reticulocalbin 3, also known as RCN3, is a member of the CREC (cab45/reticulocalbin/ ERC45/calumenin) family. RCN3 contains five Arg-Xaa-Xaa-Arg motifs, which function as target sequences of SPCs (subtilisin-like proprotein convertases), a family of serine endoproteases that proteolytically activate proproteins. The synthesis of one such member, PACE4 (paired basic amino acid cleaving enzyme 4), is influenced by association and coexpression with reticulocalbin-3. Recombinant human RCN3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Reticulocalbin 3, RLP49
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
37.9 kDa (333aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Teglund S., et al. (1994) Genomics. 23:669-684.; ; Tsuji A., et al. (2006) Biochem J. 396:51-59.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_065701
Uniprot Accession Number
Q96D15
Species
Human
AA Sequence
Frequently Asked Questions
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