Recombinant human CTDSPL protein
CTDSPL, also known as CTD small phosphatase-like protein, preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Recombinant human CTDSPL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
CTD small phosphatase-like protein, HYA22, PSR1, RBSP3, SCP3, CTD small phosphatase like protein
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
23.9 kDa (209aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Choudhary C., et al. (2009) Science. 325:834-840; ; Daub H., et al. (2008) Mol. Cell. 31:438-448
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_005799
Uniprot Accession Number
O15194
Species
Human
AA Sequence
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