Recombinant human PTPMT1 protein

PTPMT1 (protein tyrosine phosphatase mitochondrial 1), also known as MOSP or PLIP (phosphoinositide lipid phosphatase) and previously known as DuSP23, is a widely expressed PTP membrane protein with high expression levels in pancreatic beta cells. This protein exclusively localizes to the matrix face of the inner membrane of the mitochondrion. It is responsible for dephosphorylating mitochondrial proteins and therefore plays a significant role in the production of ATP and secretion of insulin. For its substrate, PTPMT1 displays a specific preference for the lipid signaling molecule phosphatidylinositol 5-phosphate. Recombinant human PTPMT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Potein tyrosine phosphatase mitochondrial 1, Phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1, PTEN-like phosphatase, Phosphoinositide lipid phosphatase, MOSP, PLIP, DUSP23

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.5 kDa (199aa) confirmed by MALDI-TOF

Additionnal Information

PTPMT1, Protein tyrosine phosphatase mitochondrial 1, Protein tyrosine phosphatase, mitochondrial 1, DuSP23, FLJ46081, MOSP, PLIP, PNAS-129, ATGP1353-10 µg, ATGP1353-20 µg, ATGP1353-50 µg, ATGP1353-100 µg, ATGP1353-250 µg, ATGP1353-500 µg, ATGP1353-1 mg, ATGP1353-10, ATGP1353-20, ATGP1353-50, ATGP1353-100, ATGP1353-250, ATGP1353-500, ATGP1353-1

References & Citations

Pagliarini DJ., et al. (2005) Mol Cell. 19 (2) :197-207.; ; Merlot S., et al. (2003) J Biol Chem. 278 (41) :39866-73.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_783859.1

Uniprot Accession Number

Q8WUK0

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHM>KVPGR AHRDWYHRID PTVLLGALPL RSLTRQLVQD ENVRGVITMN EEYETRFLCN SSQEWKRLGV EQLRLSTVDM TGIPTLDNLQ KGVQFALKYQ SLGQCVYVHC KAGRSRSATM VAAYLIQVHK WSPEEAVRAI AKIRSYIHIR PGQLDVLKEF HKQITARATK DGTFVISKT