Recombinant human VAT1 protein
VAT1 (Synaptic vesicle membrane protein VAT-1 homolog) belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins. Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. This protein increased expression in glioblastomas and on wounding, in basal keratinocytes. This expression is calcium ion-dependent. Recombinant human VAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Synaptic vesicle membrane protein VAT-1 homolog, VATI, FLJ20230, vesicle amine transport protein 1 homolog
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
44.1 kDa (413aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Koch J., et al. (2003) Arch. Dermatol. Res. 295:203-210; ; Mertsch S., et al. (2009) Neuropathol. Appl. Neurobiol. 35:342-352
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl
Scientific Category
Neuroscience
NCBI Accession Number
NP_006364
Uniprot Accession Number
Q99536
Species
Human
AA Sequence
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