Recombinant human PP1 Catalytic protein

PPP1CA, also known as serine/threonine-protein phosphatase PP1-alpha catalytic subunit, is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. This protein may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Recombinant human PPP1CA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10 mM of substrate.

Product Name Alternative

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PP1alpha, PPP1A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.2 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Bioactivity

Specific activity: >3,000unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10 mM of substrate.

Molecular Weight

39.7 kDa (350aa) confirmed by MALDI-TOF

Additionnal Information

PP1 Catalytic, Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PP1alpha, PPP1A, ATGP1174-10 µg, ATGP1174-20 µg, ATGP1174-50 µg, ATGP1174-100 µg, ATGP1174-250 µg, ATGP1174-500 µg, ATGP1174-1 mg, ATGP1174-10, ATGP1174-20, ATGP1174-50, ATGP1174-100, ATGP1174-250, ATGP1174-500, ATGP1174-1

References & Citations

He B., et al. (1997) Proc. Natl. Acad. Sci. u.S.A. 94:843-848.; ; Trinkle-Mulcahy L., et al. (2001) J. Cell Sci. 114:4219-4228.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 50 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2, 50%glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_002699.1

Uniprot Accession Number

P62136

Species

Human

AA Sequence

MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK