Recombinant human PA1/PAGR1 protein

PA1 is a component of a Set1-like multiprotein histone methyltransferase complex. It interacts with PAXIP1/PTIP; the interaction is direct and is required for the association with the rest of the PTIP complex. This protein plays a critical role in maintaining genome stability, condensation of chromatin and progression through mitosis. Recombinant human PA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

PAXIP1 associated glutamate rich protein 1, Glutamate-rich coactivator interacting with SRC1/NCOA1, PTIP-associated 1 protein, Glutamate-rich coactivator associated with SRCC16orf53, GAS PA1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

29.9 kDa (274aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

PA1, PAXIP1-associated protein 1., FLJ22459, GAS, MGC4606, C16orf53., ATGP1058-10 µg, ATGP1058-20 µg, ATGP1058-50 µg, ATGP1058-100 µg, ATGP1058-250 µg, ATGP1058-500 µg, ATGP1058-1 mg, ATGP1058-10, ATGP1058-20, ATGP1058-50, ATGP1058-100, ATGP1058-250, ATGP1058-500, ATGP1058-1

References & Citations

Liang J., et al. (2009) EMBO Rep. 10 (1) :51-7. ; ; Cho YW., et al. (2007) J Biol Chem. 282 (28) :20395-406.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_078792

Uniprot Accession Number

Q9BTK6

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSLARGHGDT AASTAAPLSE EGEVTSGLQA LAVEDTGGPS ASAGKAEDEG EGGREETERE GSGGEEAQGE VPSAGGEEPA EEDSEDWCVP CSDEEVELPA DGQPWMPPPS EIQRLYELLA AHGTLELQAE ILPRRPPTPE AQSEEERSDE EPEAKEEEEE KPHMPTEFDF DDEPVTPKDS LIDRRRTPGS SARSQKREAR LDKVLSDMKR HKKLEEQILR TGRDLFSLDS EDPSPASPPL RSSGSSLFPR QRKY