TruScript First Strand cDNA Synthesis Kit

Convenient – With the ready-to-use Master Mix, the user needs only to add template to the master mix and enzyme in order to set up the reverse transcriptase reaction Time Savings - Set up RT reactions in a shorter time since less pipetting steps are required Cost Efficient – No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix kits High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products Robust Enzyme – broad range of working temperatures from 37°C to 60°C. Capable of amplifying difficult templates with a high degree of reproducibility TruScript Reverse Transcriptase is Available in Three Convenient Formats: TruScript Reverse Transcriptase Kit (Cat.#54440) This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL) . This enzyme can be used for reverse-transcription reactions with any user-supplied primers. TruScript First Strand cDNA Synthesis Kit (Cat.#54420) This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts) . The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400) This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts) . The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.