Recombinant human MKK3 protein

MAP2K3, also known as dual specificity mitogen-activated protein kinase kinase 3, is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Recombinant human MAP2K3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Dual specificity mitogen-activated protein kinase kinase 3, MAPKK3, MEK3, MKK3, PRKMK3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

38.3 kDa (338aa) confirmed by MALDI-TOF

Additionnal Information

MKK3, Dual specificity mitogen-activated protein kinase kinase 3, MAPKK3, MEK3, MKK3, PRKMK3, ATGP0999-10 µg, ATGP0999-20 µg, ATGP0999-50 µg, ATGP0999-100 µg, ATGP0999-250 µg, ATGP0999-500 µg, ATGP0999-1 mg, ATGP0999-10, ATGP0999-20, ATGP0999-50, ATGP0999-100, ATGP0999-250, ATGP0999-500, ATGP0999-1

References & Citations

Rampoldi L., et al. (1997) Cytogenet Cell Genet. 78 (3-4) :301-3.; ; Barr AJ., et al. (2002) Biochem Biophys Res Commun. 293 (1) :647-52.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_002747.2

Uniprot Accession Number

P46734

Species

Human

AA Sequence

MSKPPAPNPT PPRNLDSRTF ITIGDRNFEV EADDLVTISE LGRGAYGVVE KVRHAQSGTI MAVKRIRATV NSQEQKRLLM DLDINMRTVD CFYTVTFYGA LFREGDVWIC MELMDTSLDK FYRKVLDKNM TIPEDILGEI AVSIVRALEH LHSKLSVIHR DVKPSNVLIN KEGHVKMCDF GISGYLVDSV AKTMDAGCKP YMAPERINPE LNQKGYNVKS DVWSLGITMI EMAILRFPYE SWGTPFQQLK QVVEEPSPQL PADRFSPEFV DFTAQCLRKN PAERMSYLEL MEHPFFTLHK TKKTDIAAFV KEILGEDS