Recombinant human ACYP1 protein
ACYP1, also known as erythrocyte acylphosphatase, is a cytosolic enzyme that catalyzes the hydrolysis of the carboxyl-phosphate bond of acylphosphates. Two acylphophatase isoenzymes exist: ACYP1 and ACYP2. The two isoenzymes share 60% homology and have the same substrate specificity, although ACYP1 has higher catalytic activity than ACYP2. Recombinant human ACYP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Acylphosphatase-1, ACYPE, erythrocyte acylphosphatase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by BCA assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
13.6 kDa (122aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Fiaschi T, et al. (1995) . FEBS Lett. 367:145-8.; ; Paoli P., et al. (2003) . J. Biol. Chem. 278: 194-199.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_001098
Uniprot Accession Number
P07311
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAEGNTL ISVDYEIFGK VQGVFFRKHT QAEGKKLGLV GWVQNTDRGT VQGQLQGPIS KVRHMQEWLE TRGSPKSHID KANFNNEKVI LKLDYSDFQI VK
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