Recombinant human DDIT4 protein

DDIT4, also known as Dig2 or REDD1, is thought to have function in the regulation of reactive oxygen species. In response to stress due to DNA damage and glucocorticoid treatment, DDIT4 is upregulated at the transcriptional level. DDIT4 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. Recombinant human DDIT4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DNA damage-inducible transcript 4 protein, Dig2, FLJ20500, REDD1, RP11-442H21.1, RTP801

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.5 kDa (252aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

DDIT4, DNA damage-inducible transcript 4 protein, Dig2, FLJ20500, REDD1, RP11-442H21.1, RTP801, ATGP0896-10 µg, ATGP0896-20 µg, ATGP0896-50 µg, ATGP0896-100 µg, ATGP0896-250 µg, ATGP0896-500 µg, ATGP0896-1 mg, ATGP0896-10, ATGP0896-20, ATGP0896-50, ATGP0896-100, ATGP0896-250, ATGP0896-500, ATGP0896-1

References & Citations

Yoshida T., et al. (2010) Nat Med. 16 (7) :767-73.; ; Regazzetti C., et al. (2010) J Biol Chem. 285 (8) :5157-64.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_061931

Uniprot Accession Number

Q9NX09

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC