Recombinant human DNAJB2 protein

DnaJB2, also known as HSJ1 or HSPF3, is belongs to the DnaJ family. It is expressed almost exclusively in the brain, with the highest levels in the frontal cortex and hippocampus. The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions. The DnaJ proteins play a critical role in the HSP70 chaperone machine by interacting with HSP70 to stimulate ATP hydrolysis. DnaJB2 is important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. Recombinant human DNAJB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DnaJ homolog subfamily B member 2, HSJ1, HSPF3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

33 kDa (300aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

DNAJB2, DnaJ homolog subfamily B member 2, HSJ1, HSPF3, ATGP0788-10 µg, ATGP0788-20 µg, ATGP0788-50 µg, ATGP0788-100 µg, ATGP0788-250 µg, ATGP0788-500 µg, ATGP0788-1 mg, ATGP0788-10, ATGP0788-20, ATGP0788-50, ATGP0788-100, ATGP0788-250, ATGP0788-500, ATGP0788-1

References & Citations

Stewart GR., et al. (2004) Tuberculosis. 84 (3-4) :180-7. ; ; Tomoyasu T., et al. (1998) Mol Microbiol. 30 (3) :567-81.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_001034639

Uniprot Accession Number

P25686

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMASYYEI LDVPRSASAD DIKKAYRRKA LQWHPDKNPD NKEFAEKKFK EVAEAYEVLS DKHKREIYDR YGREGLTGTG TGPSRAEAGS GGPGFTFTFR SPEEVFREFF GSGDPFAELF DDLGPFSELQ NRGSRHSGPF FTFSSSFPGH SDFSSSSFSF SPGAGAFRSV STSTTFVQGR RITTRRIMEN GQERVEVEED GQLKSVTING VPDDLALGLE LSRREQQPSV TSRSGGTQVQ QTPASCPLDS DLSEDEDLQL AMAYSLSEME AAGKKPADVF