BNIP-3 rabbit pAb

This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014],

Product Specifications

Background

Product Name Alternative

BNIP3; NIP3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

UniProt

Q12983

Swiss Prot

Q12983

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human BNIP3. AA range:71-120

Target

BNIP-3

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

22kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

22kD

Fragment

IgG

Subcellular Location

Mitochondrion. Mitochondrion outer membrane; Single-pass membrane protein. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane.