Recombinant human MMAB protein
MMAB is a protein that catalyzes the final step in the conversion of vitamin B (12) into adenosylcobalamin (AdoCbl), a vitamin B12 containing coenzyme for methylmalonyl-CoA mutase (MCM) . Impaired MMAB activity leads to the inherited disorder vitamin B12 dependent methylmalonic aciduria linked to the cblB complementation group. Recombinant human MMAB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
26.3 kDa (239aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gravel RA., et al. (2009) Mol Genet Metab. 98 (3) :278-84; ; Edwards AM., et al (2004) J Biol Chem. 279 (22) :23646-53.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
AAH05054
Uniprot Accession Number
Q96EY8
Species
Human
AA Sequence
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