Recombinant E.coli trxA protein

Thioredoxin 1 (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant E. coli TXN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >70 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Product Name Alternative

Thioredoxin 1, TrxA1, TrxA2

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is >70 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Molecular Weight

12.8 kDa (117aa) confirmed by MALDI-TOF

Additionnal Information

Thioredoxin1, dasC, fipA, tsnC, ATGP0549-10 µg, ATGP0549-20 µg, ATGP0549-50 µg, ATGP0549-100 µg, ATGP0549-250 µg, ATGP0549-500 µg, ATGP0549-1 mg, ATGP0549-010, ATGP0549-020, ATGP0549-050, ATGP0549-100, ATGP0549-250, ATGP0549-500, ATGP0549-01M