Recombinant human FKBP25/FKBP3 protein

FK506 binding protein 3 (FKBP3), also known as FKBP25, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP3 associates with transcriptional repressor protein YY1 and histone deaceltylases, HDAC1 and HDAC2. Also, FKBP3 may contain several casein kinase II phosphorylation sites, which are believed to be important for cell growth regulation. It is localized in the nucleus and is expressed in the brain, testis, ovary, and spleen. Recombinant human FKBP3 was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Product Name Alternative

FKBP prolyl isomerase 3, Peptidyl-prolyl cis-trans isomerase FKBP3, PPIase FKBP3, 25 kDa FK506-binding protein, 25 kDa FKBP, FKBP-25, FK506-binding protein 3, FKBP-3, Immunophilin FKBP25, Rapamycin-selective 25 kDa immunophilin, Rotamase

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Molecular Weight

25.1 kDa (224aa) confirmed by MALDI-TOF

Additionnal Information

Rotamase, Rapamycin-selective 25kDa immunophilin, Rapamycin-selective 25 kDa immunophilin, Rapamycin selective 25kDa immunophilin, Rapamycin selective 25 kDa immunophilin, PPIase FKBP3, PPIase, Peptidyl-prolyl cis-trans isomerase FKBP3, Peptidyl prolyl cis trans isomerase FKBP3, Immunophilin FKBP25, FKBP-3, FKBP3, FKBP-25, FKBP25, FKBP prolyl isomerase 3, FKBP 3, FKBP 25, FK506-binding protein 3, FK506 binding protein3, FK506 binding protein 3, 25kDa FKBP, 25kDa FK506 binding protein, 25 kDa FKBP, 25 kDa FK506-binding protein, 25 kDa FK506 binding protein, ATGP0494-10 µg, ATGP0494-20 µg, ATGP0494-50 µg, ATGP0494-100 µg, ATGP0494-250 µg, ATGP0494-500 µg, ATGP0494-1 mg, ATGP0494-010, ATGP0494-020, ATGP0494-050, ATGP0494-100, ATGP0494-250, ATGP0494-500, ATGP0494-01M

References & Citations

Yang WM., et al. (2001) EMBO J. 20 (17) :4814-25. ; ; Jin YJ., et al. (1993) Proc Natl Acad Sci u S A. 90 (16) :7769-73. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_002004

Uniprot Accession Number

Q00688

Species

Human

AA Sequence

MAAAVPQRAW TVEQLRSEQL PKKDIIKFLQ EHGSDSFLAE HKLLGNIKNV AKTANKDHLV TAYNHLFETK RFKGTESISK VSEQVKNVKL NEDKPKETKS EETLDEGPPK YTKSVLKKGD KTNFPKKGDV VHCWYTGTLQ DGTVFDTNIQ TSAKKKKNAK PLSFKVGVGK VIRGWDEALL TMSKGEKARL EIEPEWAYGK KGQPDAKIPP NAKLTFEVEL VDID