Recombinant human PDCD6IP protein

Alix, also known as PDCD6IP, is a cytoplasmic protein that interacts with apoptosis-associated proteins (ALG-2 and PDCD6) and with the endocytosis-regulator CIN85. It's involved in concentration and sorting of cargo proteins of the multivesicular body for incorporation into intralumenal vesicles that are generated by invagination and scission from the limiting membrane of the endosome. Overexpression of this protein and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death. Recombinant PDCD6IP protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Programmed cell death 6 interacting protein, PDCD6-interacting protein, ALG-2 interacting protein X, ALG-2-interacting protein 1, Alix, AIP1, Hp95

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

45.8 kDa (412aa) confirmed by MALDI-TOF

Additionnal Information

Alix, ATGP0482-10 µg, ATGP0482-20 µg, ATGP0482-50 µg, ATGP0482-100 µg, ATGP0482-250 µg, ATGP0482-500 µg, ATGP0482-1 mg, ATGP0482-10, ATGP0482-20, ATGP0482-50, ATGP0482-100, ATGP0482-250, ATGP0482-500, ATGP0482-1

References & Citations

Morita E., et al. (2007) EMBO J. 26 (19) :4215-27. ; ; Katoh K., et al. (2004) Arch Biochem Biophys. 421 (1) :159-65.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Scientific Category

Apoptosis

NCBI Accession Number

NP_037506.2

Uniprot Accession Number

Q8WUM4

Species

Human

AA Sequence

MATFISVQLK KTSEVDLAKP LVKFIQQTYP SGGEEQAQYC RAAEELSKLR RAAVGRPLDK HEGALETLLR YYDQICSIEP KFPFSENQIC LTFTWKDAFD KGSLFGGSVK LALASLGYEK SCVLFNCAAL ASQIAAEQNL DNDEGLKIAA KHYQFASGAF LHIKETVLSA LSREPTVDIS PDTVGTLSLI MLAQAQEVFF LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDTLPKE VFPVLAAKHC IMQANAEYHQ SILAKQQKKF GEEIARLQHA AELIKTVASR YDEYVNVKDF SDKINRALAA AKKDNDFIYH DRVPDLKDLD PIGKATLVKS TPVNVPISQK FTDLFEKMVP VSVQQSLAAY NQRKADLVNR SIAQMREATT LA