K562 Lysate
K-562 is an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. Cells are also used for haemoglobin synthesis, antitumour testing, tumorigenicity, differentiation, pharmacodynamics, cloning, cytotoxicity, cell biology, cellular effects of hyperthermia, and natural killer assays.
Product Specifications
Antigen Species
Human
Applications
WB
Concentration
2 mg/mL (determined by BCA assay)
Additionnal Information
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Scientific Category
Lysates
Species
Human
WB Description
All lanes : K562 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)
Preparation
Heat lysate to 95°C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody
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