NFYB cDNA

The protein encoded by NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. NFYB product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.

Product Specifications

Product Name Alternative

CBF-A, CBF-B, HAP3, NF-YB

Chromosomal Location

12q22-q23

Antigen Species

Human

Vector Type

PATGen (puc19-derived cloning vector)

Sequence

ATGACAATGGATGGTGACAGTTCTACAACAGATGCTTCTCAACTAGGAATCTCTGCAGACTATATTGGAGGAAGTCATTATGTTATACAGCCTCATGATGATACTGAGGACAGCATGAATGATCATGAAGACACAAATGGTTCAAAAGAAAGTTTCAGAGAACAAGATATATATCTTCCAATAGCAAACGTGGCTAGGATAATGAAAAATGCCATACCTCAAACGGGAAAGATTGCAAAAGATGCCAAAGAATGTGTTCAAGAATGTGTAAGTGAGTTCATCAGTTTTATAACATCTGAAGCAAGTGAAAGGTGCCATCAAGAGAAACGGAAAACAATCAATGGAGAAGATATTCTCTTTGCTATGTCTACTTTAGGCTTTGACAGTTATGTGGAACCTCTGAAATTATACCTTCAGAAATTCAGAGAGGCTATGAAAGGAGAAAAGGGAATTGGTGGAGCAGTCACAGCTACAGATGGACTAAGTGAAGAGCTTACAGAGGAGGCATTTACTAACCAGTTACCAGCTGGCTTAATAACCACAGACGGTCAACAACAAAATGTTATGGTTTACACAACATCATATCAACAGATTTCTGGTGTTCAGCAAATTCAGTTTTCATGA

Additionnal Information

NFYB, CBF-A, CBF-B, HAP3, NF-YB, NFYB, ATGD0258-10 µg, ATGD0258-20 µg, ATGD0258-50 µg, ATGD0258-100 µg, ATGD0258-250 µg, ATGD0258-500 µg, ATGD0258-1 mg, ATGD0258-10, ATGD0258-20, ATGD0258-50, ATGD0258-100, ATGD0258-250, ATGD0258-500, ATGD0258-1

Storage Conditions

Store the plasmid at -20C.

Formulation

Lyophilized

Scientific Category

Signal Transduction

Omim

189904

NCBI Accession Number

NP_006157.1

Species

Human

Preparation

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

RNA Reference

NM_006166.3

DNA Size

624bp

Vector Description

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.