Anti-Choline Acetyltransferase (ChAT) Antibody

This antipeptide antibody was generated in chickens against a sequence shared between the mouse (Q03059) and human (P28329) gene products. The antibody was affinity-purified, and its concentration was adjusted to 100 μg/mL. This antibody has been validated with human, mouse and rat tissues.

Product Specifications

Background

Murine choline-O-acetyltransferase (EC 2.3.1.6) is a 71,721 dalton protein (640 amino acids) expressed in cholinergic neurons of both the PNS and CNS. In the CNS, ChAT is expressed in motor neurons and pre-ganglionic autonomic neurons of the spinal cord, a subset of neurons in the neostriatum, and in the basal forebrain. In the PNS, ChAT is expressed in a small subpopulation of sympathetic neurons and in all parasympathetic neurons. ChAT is the enzyme responsible for synthesis of acetylcholine from acetyl-coenzyme A and choline.

Synonyms

Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, EC 2.3.1.6

Gene Name

CHAT

UniProt

P28329

Host

Chicken

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide

Target

Murine choline-O-acetyltransferase (EC 2.3.1.6)

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Cell Markers / Neuronal Markers / Cholinergic Neuronal Markers

Concentration

100 μg/mL

Format

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

72 kDa

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Notes

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the Choline Acetyltransferase gene product shared between the human (P28329, NCBI) and mouse (Q03059, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentrations of the eluates were then adjusted to 100 μg/mL, and the preparation was filter-sterilized.

IHC Dilution

1:1000-1:2000

WB Dilution

Not Recommended

ICC Dilution

1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.