MERS-CoV Spike RBD antibody

MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses. MERS-CoV was first identified in the Kingdom of Saudi Arabia in 2012, which is a single and positive stranded RNA virus. Dromedary camels are widely considered as the source of the transmission of MERS-CoV. The rate of human transmission among household contacts of MERS patients has been approximately 5 % based on serological analysis. MERS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. MERS-CoV S mediates viral attachment and fusion to human cells via human cellular receptor DPP4, also known as CD26. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Specifications

Background

ELISA: MERS Spike RBD Antibody (1 µg/mL) specifically recognizes MERS Spike, Spike S1 and Spike RBD recombinant protein, but not interacted MERS Spike S2 recombinant protein in ELISA.

Product Name Alternative

Middle East respiratory syndrome coronavirus, Human betacoronavirus 2c EMC/2012, MERS-CoV, MERS, MERS-CoV RBD, MERS RBD, receptor binding domain, RBD, Spike RBD protein

Host

Mouse

Antigen Species

MERS-CoV

Reactivity

MERS-CoV

Immunogen

Recombinant MERS-CoV Spike RBD (358-606aa) purified from Baculovirus

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT2F7

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Covid-19, covid19, sars, sars2, ATGA0595-10 µg, ATGA0595-20 µg, ATGA0595-50 µg, ATGA0595-100 µg, ATGA0595-250 µg, ATGA0595-500 µg, ATGA0595-1 mg, ATGA0595-10, ATGA0595-20, ATGA0595-50, ATGA0595-100, ATGA0595-250, ATGA0595-500, ATGA0595-1

References & Citations

Junghyun Goo., et al. (2020) Virus Res. 278:197863. ; ; Yan-Hua Li., et al. (2019) Engineering. 5:940-947.; ; Lingshu Wang., et al. (2018) J Virol. 92:e02002-2017. ; ; Nicolas Papageorgiou., et al. (2016) Acta Crystallogr D Struct Biol. 72:192-202.; ; Xiao-Yan Che., et al. (2004) J Clin Microbiol. 42:2629-2635.;

Other References

Lee K, et al. Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus. (Microbiol Immunol. 2018) {https://pubmed.ncbi.nlm.nih.gov/30117617/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Virus

NCBI Accession Number

AFS88936

Uniprot Accession Number

K0BRG7

WB Description

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MERS Spike RBD antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant MERS Spike protein (ATGP3979) ; ; Lane 2.: Recombinant MERS Spike S1 protein (ATGP3980) ; ; Lane 3.: Recombinant MERS Spike S2 protein (ATGP3981) ; ; Lane 4.: Recombinant MERS Spike RBD protein (ATGP3982) ;