Human NM23-H2/NME2 antibody

NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways.

Product Specifications

Product Name Alternative

NME/NM23 nucleoside diphosphate kinase 2, Nucleoside diphosphate kinase B, C-myc purine-binding transcription factor PUF, Histidine protein kinase NDKB, NDK B, NDP kinase B, NM23B, NDPKB

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NME2 (1-152aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT2E3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NM23-H2, ATGA0475-10 µg, ATGA0475-20 µg, ATGA0475-50 µg, ATGA0475-100 µg, ATGA0475-250 µg, ATGA0475-500 µg, ATGA0475-1 mg, ATGA0475-10, ATGA0475-20, ATGA0475-50, ATGA0475-100, ATGA0475-250, ATGA0475-500, ATGA0475-1

References & Citations

Munkonge FM., et al. (2009) J Biol Chem. 284 (39) : 26978-87.; ; Treharne KJ., et al. (2009) FEBS Lett. 583 (17) : 2789-92.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001018149

Uniprot Accession Number

P22392

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: A549 cell lysate; ; Lane 3.: MCF7 cell lysate; ; Lane 4.: LnCap cell lysate The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: NME2 transfected 293T cell lysate {ATGA0475-WB2.jpg}

IF Description

ICC/IF analysis of NME2 in HeLa cells. The cell was stained with ATGA0475 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .