Human PIN/DYNLL1 antibody

The LC8 family members of dynein light chains (DYNLL1 and DYNLL2 in vertebrates) are highly conserved ubiquitous eukaryotic homodimer proteins that interact, besides dynein and myosin 5a motor proteins, with a large (and still incomplete) number of proteins involved in diverse biological functions.

Product Specifications

Product Name Alternative

Dynein light chain 1 cytoplasmic, DLC1, DLC8, DNCL1, DNCLC1, hdlc1, LC8, LC8a, MGC126137, MGC126138, PIN, 8 kDa dynein light chain, Dynein light chain LC8-type 1, Protein inhibitor of neuronal nitric oxide synthase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human DYNLL (1-89aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT13C9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PIN, DYNLL, Dynein light chain 1 cytoplasmic, DLC1, DLC8, DNCL1, DNCLC1, hdlc1, LC8, LC8a, MGC126137, MGC126138, PIN, ATGA0383-10 µg, ATGA0383-20 µg, ATGA0383-50 µg, ATGA0383-100 µg, ATGA0383-250 µg, ATGA0383-500 µg, ATGA0383-1 mg, ATGA0383-10, ATGA0383-20, ATGA0383-50, ATGA0383-100, ATGA0383-250, ATGA0383-500, ATGA0383-1

References & Citations

Rapali P., et al. (2011) FEBS J. 278 (17) : 2980-2996.; ; Radnai L., et al. (2010) J Biol Chem. 285 (49) : 38649-38657.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_003737

Uniprot Accession Number

P63167

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DYNLL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : A549 cell lysate ; ; Lane 3. : 293T cell lysate ; ; Lane 4. : HepG2 cell lysate

IF Description

ICC/IF analysis of DYNLL in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DYNLL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .