Human alpha-Smooth Muscle ActiCTA2 antibody

Alpha-actin-2 also known as actin, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA) . Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic beta- and gamma-actin proteins are referred to as non-muscle actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. Actin has major functions. Firstly, F-actin polymers form microfilaments - polar intracellular tracks for kinesin motor proteins, allowing the transport of vesicles, organelles and other cargo. Actin is a component of the cytoskeleton and links to alpha-actinin, E-cadherin and beta-catenin at adherens junctions. actin has a role in cell motility through polymerization and depolymerization of fibrils.

Product Specifications

Product Name Alternative

Aortic smooth muscle actin, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, Actin, Aortic smooth muscle, Intermediate form, ACTSA, ACTVS, GIG46

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ACTA2 (3-377aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT132D3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Aortic smooth muscle actin, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, Actin, Aortic smooth muscle, Intermediate form, ACTSA, ACTVS, GIG46, Alpha actin 2, Alpha actin2, Cell growth inhibiting gene 46 protein, ATGA0358-10 µg, ATGA0358-20 µg, ATGA0358-50 µg, ATGA0358-100 µg, ATGA0358-250 µg, ATGA0358-500 µg, ATGA0358-1 mg, ATGA0358-010, ATGA0358-020, ATGA0358-050, ATGA0358-100, ATGA0358-250, ATGA0358-500, ATGA0358-01M

References & Citations

Herman IM. et al. (1993) Curr Opin Cell Biol. 5 (1) : 48-55.; ; Guo DC., et al. (2009) Am J Hum Genet. 84 (5) : 617-627.

Other References

Liu X, et al. Human aortic valve interstitial cells obtained from patients with aortic valve stenosis are vascular endothelial growth factor receptor 2 positive and contribute to ectopic calcification. (J Pharmacol Sci. 2021) {https://pubmed.ncbi.nlm.nih.gov/33451756/}; ; Sakata N, et al. Optimal temperature for the long-term culture of adult porcine islets for xenotransplantation. (Front Immunol. 2023) {https://pubmed.ncbi.nlm.nih.gov/37901206/}; ; Men S, et al. Role of CD34 in calcification of human aortic valve interstitial cells from patients with aortic valve stenosis. (J Pharmacol Sci.2024) {https://pubmed.ncbi.nlm.nih.gov/39313278/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Actin & Myosin Related

NCBI Accession Number

NP_001604

Uniprot Accession Number

P62736

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTA2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: SW480 cell lysate; ; Lane 3.: HeLa cell lysate

FACS Description

Flow cytometry analysis of ACTA2 in Nb2-11 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of ACTA2 in HeLa cells. The cell was stained with ATGA0358 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .