Human Epoxide Hydrolase 1/EPHX1 antibody

Epoxide hydrolase 1 (EPHX1) is a family of biotransformation enzyme which is involved in the hydrolysis of various epoxides and epoxide intermediates. Epoxide hydrolase plays an important role in both the activation and detoxification of exogenous chemicals such as polycyclic aromatic hydrocarbons.

Product Specifications

Product Name Alternative

Epoxide hydrolase 1, Epoxide hydrolase 1 microsomal (xenobiotic), MEH, EPHX, EPOX

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human EPHX1 (21-455aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT2E5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Microsomal (xenobiotic), MEH, Epoxide hydrolase 1 microsomal (xenobiotic), Epoxide hydrolase 1, EPOX, EPHX1, EPHX, ATGA0143-10 µg, ATGA0143-20 µg, ATGA0143-50 µg, ATGA0143-100 µg, ATGA0143-250 µg, ATGA0143-500 µg, ATGA0143-1 mg, ATGA0143-010, ATGA0143-020, ATGA0143-050, ATGA0143-100, ATGA0143-250, ATGA0143-500, ATGA0143-01M

References & Citations

Bertvan Loo, et al., (2006) Appl Environ Microbiol 72 (4) :2905-2917.; ; Janet K.C, et al., (2001) The Histochemical Journal 33:329-336.; ; Hassett C, et al., (1994) Genomics 23 (2) :433-442.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000111

Uniprot Accession Number

P07099

WB Description

The tissue lysate (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHX1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Liver Tissue lysateThe cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHX1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: EPHX1 Transfected 293T cell lysate; ; {ATGA0143-WB2.jpg}

IF Description

ICC/IF analysis of EPHX1 in Hep3B cells. The cell was stained with ATGA0143 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .