Human FABP4/A-FABP antibody

FABP4 (Fatty acid binding protein 4) is expressed in adipocytes and macrophages, and integrates inflammatory and metabolic responses. FABP4 is thought to regulate fatty acid uptake, release, and storage in adipocytes and participates in systemic glucose homeostasis and in macrophage responses in atherosclerosis. Blocking this protein either through genetic engineering or drugs has the possibility of treating heart disease, diabetes, asthma, obesity, and fatty liver disease.

Product Specifications

Product Name Alternative

Fatty acid binding protein 4, fatty acid binding protein 4 adipocyte, A-FABP, aP2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human FABP4 (1-132aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

3F4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

IL-1A, IL1A, IL-1 alpha, IL1 alpha, IL 1A, IL 1 alpha, IL 1 A, Hematopoietin-1, Hematopoietin1, Hematopoietin 1, Fatty acid binding protein 4 adipocyte, Fatty acid binding protein 4, FABP4, AP2, ALBP, A-FABP, AFABP, A FABP, AFA0920-10 µg, AFA0920-20 µg, AFA0920-50 µg, AFA0920-100 µg, AFA0920-250 µg, AFA0920-500 µg, AFA0920-1 mg, AFA0920-010, AFA0920-020, AFA0920-050, AFA0920-100, AFA0920-250, AFA0920-500, AFA0920-01M

References & Citations

Furuhashi M., et al., (2007) Nature. 21.447 (7147) :959-65.; ; Shum BO, et al., (2006) J Clin Invest. 116 (8) :2183-92.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001433

Uniprot Accession Number

P15090

WB Description

The tissue lysates of mouse heart (40ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FABP4 (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The tissue lysates of mouse adipose (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP4 (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {AFA0920-WB2.jpg}

IF Description

ICC/IF analysis of FABP4 in HeLa cells. The cell was stained with AFA0920 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .