BRD4/CAP Polyclonal Antibody, AbBy Fluor™ 680 Conjugated

Product Specifications

Background

Brd4 belongs to the BET family, a group of structurally related proteins containing two bromodomains. Through these two domains, Brd4 associates with mitotic chromosomes and its expression correlates with cell growth. Expression of Brd4 inhibits cell cycle progression from G (1) to S, due to binding to the largest subunit of replication factor C (RFC) to prevent DNA elongation. Altered Brd4 function correlates with poorly differentiated carcinoma, with aggresive phenotype and a highly lethal outcome.

Synonyms

Brd4; BRD4_HUMAN; bromodomain containing protein 4; Bromodomain-containing protein 4; CAP; chromosome associated protein; HUNK1; Protein HUNK1.

Gene ID

23476

Swiss Prot

O60885

Host

Rabbit

Immunogen

65-160/1362

Target

BRD4/CAP

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 680

Source

KLH conjugated synthetic peptide derived from human Brd4/CAP

Applications

WB, IF (IHC-P)

Purification

Purified by Protein A.

Excitation Emission

679nm/702nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

23476

Predicted Cross Reactivity

Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit

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