Coronin-1B (Ser-2), Phosphospecific Antibody

Product Specifications

Background

Coronins are highly-conserved F-actin binding proteins that play important roles in lamellipodial protrusion during various types of cell motility. In yeast, coronins regulate cytoskeletal changes through inhibition of Arp2/3 complex. Human coronins have been classified in three subgroups type I (coronin-1A, -1B, -1C), type II (coronin-2A, -2B), and type III (coronin-7) . These coronins have at least one large b-propeller region that mediates protein-protein interactions and type I and II coronins have coiled-coil regions involved in oligiomerization. Coronin-1B is ubiquitously expressed and localizes to the leading edge of cell protrusions in migrating fibroblasts. Both Coronin-1B and Coronin-1A interaction with Arp2/3 complex may be regulated by phosphorylation. PKC phosphorylates the N-terminus at Ser-2 , and this phosphorylation reduces interactions with Arp2/3 leading to diminshed cell motility.

Synonyms

Coronin2, Coro1B

Swiss Prot

Q9BR76

Modification Site

Ser-2

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Coronin-1B (Ser-2)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Coronin-1B (Ser-2) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 2 in human coronin-1B.

Applications

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects a 60 kDa* band corresponding to Coronin-1B on SDS-PAGE immunoblots of human A431 or HeLa cells treated with Calyculin A. This band is not observed in control cells and the band observed in Calyculin A-treated cells can be removed with lambda phosphatase treatment. This sequence has high homology to the conserved site in rat and mouse coronin-1B and in coronin-1A. However, this sequence has low homology to the conserved region in other coronins.