Phospho-PTK9 (Tyr321) Polyclonal Antibody, Cy3 Conjugated

Product Specifications

Background

PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

Synonyms

PTK9 Tyr321; PTK9 Y321; p-PTK9 Tyr321; p-TWF1Y321; A6; A6 protein tyrosine kinase; Protein A6; Protein tyrosine kinase 9; PTK9; PTK9 protein tyrosine kinase 9; TWF 1; TWF1; Twinfilin 1; Twinfilin actin binding protein, homolog 1; Twinfilin; Twinfilin, actin-binding protein, homolog 1 Drosophila; Twinfilin, Drosophila, homolog of, 1; TWF1_HUMAN; Twinfilin-1.

Gene ID

5756

Modification Site

Tyr321

Cellular Locus

Cytoplasm

Host

Rabbit

Target

Phospho-PTK9 (Tyr321)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy3

Source

KLH conjugated synthetic phosphopeptide derived from human TWF1 around the phosphorylation site of Tyr321

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

512,550nm/570,615nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

5756

Predicted Cross Reactivity

Human, Mouse, Rat, Dog, Cow, Pig, Chicken

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