Phospho-CRK (Tyr221) Polyclonal Antibody

Product Specifications

Background

The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.

Synonyms

Adapter molecule crk; Proto-oncogene c-Crk; p38; CRK

Gene ID

1398

Modification Site

Tyr221

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Others

Target

Phospho-CRK (Tyr221)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

KLH conjugated synthetic phosphopeptide derived from human Crk p38 around the phosphorylation site of Tyr221 [GP (p-Y) AQ]

Applications

WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Gene ID URL

1398

Predicted Cross Reactivity

Human, Mouse, Rat, Dog, Horse, Chicken, Rabbit

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

MBS9411389-01 0.1 mL

PLEKHG4 antibody

Ask

View Details

MBS9411389-02 5x 0.1 mL

PLEKHG4 antibody

Ask

View Details

130696-ML490 100 µL

OGG1 (N-glycosylase/DNA Lyase, 8-oxoguanine DNA Glycosylase, DNA- (Apurinic or Apyrimidinic Site) Lyase, AP Lyase, MMH, MUTM, OGH1) (MaxLight 490)

Ask

View Details

GK9085-1kg 1 Kg

Agar, high gel strength

Ask

View Details

HY-159808-01 25 mg

TCL1

Ask

View Details

HY-159808-02 50 mg

TCL1

Ask

View Details