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Phospho-Aurora B (Tyr12) Polyclonal Antibody

Product Specifications

Background

Mahogany (MG), originally identified as a protein involved in pigmentation, acts in conjunction with melanocortin receptors to suppress diet-induced obesity. Mahogany contains a single transmembrane domain, and it is expressed in a broad range of tissues, including the hypothalamus and pigment cells. Mutations within the mahogany gene were shown to rescue agouti-lethal-yellow mutant mice from obesity. The extracellular domain of mouse mahogany is the ortholog of the human protein attractin. Attractin (also designated DPPT-L) is a human serum glycoprotein and is a member of the CUB family of cell adhesion and guidance proteins. Attractin is expressed on activated T cells and is released from the cells 48 to 72 hours after activation.

Synonyms

Aurora B phospho Y12; Aurora B phospho T12; AIK2; AIM1; ARK2; AurB; AURKB; Aurora 1; Aurora and Ipl1 like midbody associated protein 1; Aurora kinase B; Aurora related kinase 2; Aurora-B; Aurora/IPL1 related kinase 2; IPL1; Serine/theronine kinase 12; Serine/threonine protein kinase 12; STK1; STK12; STK5.

Gene ID

9212

Modification Site

Tyr12

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Mouse

Target

Phospho-Aurora B (Tyr12)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

KLH conjugated synthetic phosphopeptide derived from human Aurora B around the phosphorylation site of Tyr12

Applications

ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Gene ID URL

9212

Predicted Cross Reactivity

Human, Rat, Cow, Sheep, Pig, Horse

Curated Selection

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