SPARC Polyclonal Antibody, FITC Conjugated
Product Specifications
Background
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca (2+) with a low affinity and an EF-hand loop that binds a Ca (2+) ion with a high affinity.
Synonyms
ON; SPARC; Basement-membrane protein 4; BM-4; Osteonectin; Secreted protein acidic and rich in cysteine
Gene ID
6678
Swiss Prot
P09486
Cellular Locus
Secreted, Cell membrane, Extracellular matrix
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Immunogen
101-200/303
Target
SPARC
Clonality
Polyclonal
Isotype
IgG
Conjugation
FITC
Source
KLH conjugated synthetic peptide derived from human SPARC
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
494nm/518nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
6678
Predicted Cross Reactivity
Dog, Cow, Pig, Horse, Rabbit
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