RB1 Antibody / Retinoblastoma
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
P06400
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human Retinoblastoma recombinant protein (Position: E40-D921) was used as the immunogen for the RB1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, ICC/IF, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This RB1 antibody is available for research use only.
Storage Conditions
After reconstitution, the RB1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the RB1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Immunofluorescent staining of FFPE human A549 cells with RB1 antibody (green) and Beta Tubulin mAb (red) . HIER: steam section in pH6 citrate buffer for 20 min.
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