CYP7B1 Antibody / 25-hydroxycholesterol 7-alpha-hydroxylase
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
O75881
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human CYP7B1 recombinant protein (Position: Q127-D391) was used as the immunogen for the CYP7B1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This CYP7B1 antibody is available for research use only.
Storage Conditions
After reconstitution, the CYP7B1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the CYP7B1 antibody should be determined by the researcher.
Location
Cytoplasm (ER)
Image Legend
Immunohistochemical staining of CYP7B1 using anti-CYP7B1 antibody. CYP7B1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CYP7B1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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