CYP7B1 Antibody / 25-hydroxycholesterol 7-alpha-hydroxylase

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

O75881

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human CYP7B1 recombinant protein (Position: Q127-D391) was used as the immunogen for the CYP7B1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This CYP7B1 antibody is available for research use only.

Storage Conditions

After reconstitution, the CYP7B1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the CYP7B1 antibody should be determined by the researcher.

Location

Cytoplasm (ER)

Image Legend

Immunohistochemical staining of CYP7B1 using anti-CYP7B1 antibody. CYP7B1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CYP7B1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.