SNCA Antibody / Alpha Synuclein
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
P37840
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human Alpha Synuclein/SNCA recombinant protein (Position: M1-D135) was used as the immunogen for the SNCA antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, ICC, IF, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Limitations
This SNCA antibody is available for research use only.
Storage Conditions
After reconstitution, the SNCA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Product Datasheet
http://www.nsjbio.com/tds-pdf/snca-antibody-alpha-synuclein-fy12837-nsj-bioreagents
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the SNCA antibody should be determined by the researcher.
Location
Cytoplasmic, Nuclear
Image Legend
Immunohistochemical staining of SNCA using anti-SNCA antibody. SNCA was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SNCA antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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