SIRT6 Antibody / Sirtuin 6

Product Specifications

Specifications

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL

UniProt

Q8N6T7

Host

Rabbit

Reactivity

Human

Immunogen

E.coli-derived human SIRT6 recombinant protein (Position: D14-S355) was used as the immunogen for the SIRT6 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, FACS, IF, ICC, WB

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This SIRT6 antibody is available for research use only.

Storage Conditions

After reconstitution, the SIRT6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the SIRT6 antibody should be determined by the researcher.

Location

Nuclear, ER (minor)

Image Legend

Immunofluorescent staining of SIRT6 using anti-SIRT6 antibody (red) and anti-Beta Tubulin antibody (green) . SIRT6 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-SIRT6 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG and DyLight 488 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.