Zebrafish Dmrt1 Antibody / Doublesex and mab 3 related transcription factor 1

Doublesex and mab-3 related transcription factor 1, also known as DMRT1, is a protein which in humans is encoded by the DMRT1 gene. This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain) . The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q71MM5

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Dmrt1 recombinant protein (amino acids R44-E76) was used as the immunogen for the Zebrafish Dmrt1 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Dmrt1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Dmrt1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Dmrt1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunohistochemical analysis of Zebrafish Dmrt1 protein using Zebrafish Dmrt1 antibody and zebrafish ovary tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.