Zebrafish Sox3 Antibody / SRY-box 3

Sox3 is maternally deposited and is expressed in the neuroectoderm during early development. It acts as a transcriptional activator that promotes neural fate by regulating genes involved in progenitor cell maintenance and inhibiting premature neuronal differentiation. Sox3 also contributes to the development of the pituitary gland, sensory placodes, and hypothalamic structures. Due to its conservation across vertebrates, zebrafish Sox3 serves as a powerful model for studying neurogenesis, pluripotency, and transcriptional regulation during early embryonic development. It is also used to investigate mechanisms of cell fate decisions, stem cell biology, and congenital neurological disorders.

Product Specifications

Specifications

Western Blot: 0.5-1 µg/mL

UniProt

Q6EJB7

Host

Rabbit

Reactivity

Zebrafish

Immunogen

E. coli-derived zebrafish Sox3 recombinant protein (amino acids Q141-Q285) was used as the immunogen for the Zebrafish Sox3 antibody.

Clonality

Polyclonal

Isotype

Applications

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Sox3 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Sox3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Sox3 antibody should be determined by the researcher.

Image Legend

Western blot analysis of Sox3 protein using Zebrafish Sox3 antibody and 1) whole female zebrafish tissue lysates and 2) zebrafish embryo tissue lysates. Predicted molecular weight ~33 kDa.