CD83 Antibody

CD83 is a heavily glycosylated membrane protein of the immunoglobulin (Ig) superfamily that is expressed in mature Dendritic cells, Langerhans cells and interdigitating reticulum cells within lymphoid tissues. Structurally, CD83 resembles other Ig superfamily members, which have an extracellular V-type Ig-like domain, a single transmembrane domain and a 40 residue cytoplasmic tail. CD83 expression is used as a marker for mature, antigen presenting Dendritic cells that are capable of generating tumor-specific T cell immunity, a phenotype with implications as an anti-cancer vaccine. CD83-IgG1 (Fc) chimera studies indicate that CD83 is a sialic acid-binding, Ig-like Lectin (Siglec) adhesion molecule that is involved in cell adhesion/signaling by hosting Dendritic cell interactions with monocytes and CD8+ T cells.

Product Specifications

Specifications

Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q01151

Host

Mouse

Reactivity

Human

Immunogen

Prokaryotic recombinant protein corresponding to 124 amino acids of the external N-terminus of the CD83 molecule was used as the immunogen for the CD83 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

1H4b

Applications

WB, IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This CD83 antibody is available for research use only.

Storage Conditions

Aliquot the CD83 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CD83 antibody should be determined by the researcher.

Location

Cytoplasm, Membrane

Image Legend

Western blot testing of human RH30 and PC-3 cell lysate with CD83 antibody. Expected molecular weight: ~23-60 kDa depending on level of glycosylation.