Anti-PAK-1,2,3 (Thr402) Antibody

Our Anti-PAK-1,2,3 (Thr402) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat PAK-1,2,3 (Thr402) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs: α-PAK (also known as PAK-1) and β-PAK (also known as PAK-3) are mostly brain-specific, while γ-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003) . Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004) . PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998) . Autophosphorylation of Thr-402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000) .

Synonyms

Alpha PAK antibody, Beta PAK antibody, bPAK antibody, CDKN1A antibody, Gamma PAK antibody, hPAK3 antibody, MRX30 antibody, MRX47 antibody, Oligophrenin 3 antibody, OPHN3 antibody, P21 (CDKN1A) activated kinase 2 antibody, P21 (CDKN1A) activated kinase 3 antibody, p21 activated kinase 1 antibody, p21 activated kinase 2 antibody, p21 activated kinase 3 antibody, P21 protein (Cdc42/Rac) activated kinase 1 antibody, P21 protein (Cdc42/Rac) activated kinase 2 antibody, P21 protein (Cdc42/Rac) activated kinase 3 antibody, P21/Cdc42/Rac1 activated kinase 1 (STE20 homolog yeast) antibody, P21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody, P58 antibody, P65 PAK antibody, PAK 2 antibody, PAK 3 antibody, PAK1 antibody, PAK2 antibody, PAK3 antibody, PAK3beta antibody, PAK65 antibody, PAKalpha antibody, PAKgamma antibody, S6/H4 kinase antibody, Serine/threonine protein kinase PAK 1 antibody, Serine/threonine protein kinase PAK 2 antibody, Serine/threonine protein kinase PAK 3 antibody, Serine/threonine protein kinase PAK1 antibody, Serine/threonine protein kinase PAK2 antibody, Serine/threonine protein kinase PAK3 antibody, STE20 homolog yeast antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

PAK1-3

NCBI Gene ID

29431

UniProt

P35465

Modification Site

Thr402

Host

Rabbit

Reactivity

Mouse and Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 of rat p21 activated kinase 2 (PAK-2), conjugated to keyhole limpet hemocyanin (KLH) . The peptide sequence used is identical in PAK-1, 2 and 3. Note: Thr402 in PAK-2 corresponds to Thr423 in human PAK-1.

Immunogen Type

Rat

Target

PAK-1,2,3 (Thr402)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

68/70

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~68 kDa PAK protein phosphorylated at Thr402. The immunolabeling is completely eliminated by treatment with λ-phosphatase.