Anti-Neurofilament NF-H Antibody
Our Anti-Neurofilament NF-H primary antibody from PhosphoSolutions is chicken polyclonal. It detects all known mammal and chicken Neurofilament NF-H and is total IgY fraction. It is great for use in WB, IHC, ICC.
Product Specifications
Background
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (Harris et al., 1991) . NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (Mendonca et al., 2005) and Alzheimer's disease (Hu et al., 2002) .
Synonyms
200 kDa neurofilament protein antibody, CMT2CC antibody, Nefh antibody, Neurofilament heavy polypeptide 200kDa antibody, Neurofilament heavy polypeptide antibody, Neurofilament triplet H protein antibody, NF H antibody, NF-H antibody, NFH antibody, NFH_HUMAN antibody
Other Statements
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Gene Name
NEFH
NCBI Gene ID
4744
UniProt
P12036
Host
Chicken
Reactivity
All Known Mammal and Chicken
Immunogen
Purified bovine NF-H
Immunogen Type
Bovine
Target
Neurofilament NF-H
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
ELISA, WB, IHC, ICC
Concentration
30 mg/ml
Format
Total IgY fraction
Form
Liquid
Buffer
PBS + 10 mM NaN3.
Molecular Weight
200
Shipping Conditions
Blue Ice
Storage Conditions
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
IHC Dilution
1:5000-1:25,000
WB Dilution
1:50000
Specificity
Specific for endogenous levels of the ~200 kDa Neurofilament H protein.
Formulation
Total IgY fraction
ICC Dilution
1:25,000
Quality Control
Western blots performed on each lot.
Isotype
IgY
Curated Selection
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