GMP Recombinant Human Wnt-3a (hFc Tag)

Product Specifications

Background

Wnt surrogate is a Wnt pathway agonists and related compositions, which may be used in any of a variety of therapeutic methods for the treatment of diseases.

Product Name Alternative

MGC119418; MGC119419; MGC119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a

Accession Number

NP_149122

Host

Mammalian cell

Target

Wnt-3a (hFc Tag)

Tag

HFc Tag

Endotoxin

≤10 EU/mg

Purity

Greater than 95% as determined by reducing SDS-PAGE

Activity

Measured by its ability to induce Topflash reporterBioactivity in CHO-K1 cells. The ED50 for this effect is ＜30ng/ml.

Form

Lyophilized

Buffer

PBS, pH7.4

Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

58.2 kDa

Shipping Conditions

The product is shipped with ice bag. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

36 months at -20°C to -80°C in lyophilized state.6 months at -20°C to -80°C under sterile conditions after reconstitution.7-10 days at 2°C to 8°C under sterile conditions after reconstitution.Please minimize freeze-thaw cycles.

Construct

Recombinant Human Wnt-3a (hFc Tag) is produced by our Mammalian cell expression system and the target gene encoding Ser19-Lys352 with a C-terminal linker GGGGGS and Human lgG1 (Asp104-Lys330) is expressed.