Phospho-Lyn (Tyr507) (Clone: 5B6) rabbit mAb

Lyn, along with Btk, supports the abnormal growth and survival of neoplastic mast cells. Phosphorylated Lyn has been identified in these cancerous cells, along with phosphorylated Btk, Hck, and Stat5. Dasatinib, a chemotherapy drug used to treat leukemia, is a tyrosine kinase inihibitor that binds directly to Lyn in neoplastic cells. Lyn and Btk have also been shown to be involved in IgE receptor-dependent activation. Increased Lyn activity, detected by higher amounts of phospho Lyn, has been demonstrated in breast cancer cell lines. This is likely mediated through effects of upstream regulators of Lyn, rather than mutations in Lyn itself.

Product Specifications

Product Name Alternative

Tyrosine-protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn, JTK8

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Tyr507 of human phospho Lyn

Clonality

Monoclonal

Clone

LynY507-5B6

Applications

FACS, WB

Format

Purified

Components

1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA

Storage Conditions

Store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

1μg/mL - 0.001μg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. (0.5mg/ml, more than 200 western blots)