SARS-CoV-2 Surrogate Virus Neutralization Test Kit (KP.2)

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant RBD (KP.2) has been pre-coated onto a microplate. Standards or samples are premixed with HRP labeled ACE and then pipetted into the wells. Anti-RBD (KP.2) Neutralizing Antibody in the sample competitively binds to recombinant RBD (KP.2) with the HRP labeled ACE. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-RBD (KP.2) Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.