PD-L1 / B7-H1 / CD274 Protein (ECD, His Tag)

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1 (PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Product Specifications

Synonyms

A530045L16Rik Protein; B7h1 Protein; Cd274 Protein; Pdcd1l1 Protein; Pdcd1lg1 Protein; Pdl1 Protein

Accession Number

NP_068693.1

Reactivity

Mouse

Cross Reactivity

Mouse

Label

His-Tag

Assay Protocol

See Datasheet send with the product

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 98 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1. Loaded Recombinant Mouse PD-L1/B7-H1 Protein , His Tag on HIS1K Biosensor, can bind Recombinant Mouse PD-1 Protein, hFc Tag with an affinity constant of 0.519μM as determined in BLI assay (Sartorius Octet RED384) . 2. Recombinant Mouse PD-1 Protein , hFc Tag captured on Protein A chip can bind Recombinant Mouse PD-L1/B7-H1, His Tag with an affinity constant of 2.489 µM as determined in an SPR assay (Biacore T200) . 3. Recombinant Mouse PD-L1/B7-H1 Protein, His Tag captured on CM5 chip via Anti-his antibody, can bind Recombinant Mouse PD-1 Protein, hFc Tag with an affinity constant of 1.117 µM as determined in an SPR assay (Biacore T200) .

Length

231

Form

Lyophilized or frozen liquid

Buffer

PBS, pH 7.4

Reconstitution

See Datasheet

Molecular Weight

55 kDa (glycosylated)

Storage Conditions

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.