USP36 polyclonal antibody

Product Specifications

Background

Human USP36, encoding a DUB enzyme, was isolated from ovarian cancer cells using RT-PCR and characterized. Identified DUB enzyme activity of USP36 by analyzing its capability to cleave the ubiquitin. Structural and immunoprecipitation analyses revealed for the first time that USP36 contains the PEST motif and is polyubiquitinated.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ubiquitin carboxyl-terminal hydrolase 36; Deubiquitinating enzyme 36; Ubiquitin thioesterase 36; Ubiquitin-specific-processing protease 36; USP36; KIAA1453

Swiss Prot

Q9P275

Reactivity

Human, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 500-550 of Human USP36.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 122 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

USP36 polyclonal antibody detects endogenous levels of USP36 protein.

Applications Notes

Western blot (WB) analysis of USP36 polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug)