GAPDH Antibody [12D3D5] (biotin)

Product Specifications

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1) . GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2) . GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

NCBI Gene ID

2597

Swiss Prot

P04406

Accession Number

NP_002037

Host

Mouse

Reactivity

Human, Mouse, Rat, Rabbit

Immunogen

Biotin-GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.

Clonality

Monoclonal

Clone

12D3D5

Conjugation

Biotin

Type

Primary Antibodies

Field of Research

Signal Transduction, Neuroscience, Homeostasis

Purification

Biotin-GAPDH antibody is Protein A purified.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

1 mg/mL

Buffer

Biotin-GAPDH antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Biotin-GAPDH antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

Biotin-GAPDH antibody is human, mouse, rat, and rabbit reactive.

Symbol

GAPDH

NCBI Official Name

Glyceraldehyde-3-phosphate dehydrogenase

NCBI Organism

Homo sapiens

Background Reference 01

Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.