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BCL-2 (phospho Ser87) Antibody

Product Specifications

Background

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) .

NCBI Gene ID

596

Swiss Prot

P10415

Accession Number

NP_000624.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

BCL-2 (phospho Ser87) antibody was raised against a peptide sequence around phosphorylation site of serine 87 (A-L-S (p) -P-V) derived from Human BCL-2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

28 kDa

Fragment

IgG

Specificity

The antibody detects endogenous level of BCL-2 only when phosphorylated at serine 87.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

BCL2

NCBI Official Name

B-cell CLL/lymphoma 2

NCBI Organism

Homo sapiens

Background Reference 01

Nencioni L, et al. (2009) J Biol Chem 284, 16004-15. Pattingre S, et al. (2009) J Biol Chem 284, 2719-28. Wei Y, et al. (2008) Mol Cell 30, 678-88.

Other Product Names

Apoptosis regulator Bcl-2, BCL2

Tested Applications

WB

Protein ID

72198189

Physical Properties

Liquid

Frequently Asked Questions

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