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B-Raf (phospho Thr753) Antibody

Product Specifications

Background

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

NCBI Gene ID

673

Swiss Prot

P15056

Accession Number

NP_004324.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

B-Raf (phospho Thr753) antibody was raised against a peptide sequence around phosphorylation site of threonine 753 (P-K-T (p) -P-L) derived from Human B-Raf.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

84 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 753

Applications Notes

Western Blot: 1:500~1:1000

Symbol

BRAF

NCBI Official Name

V-raf murine sarcoma viral oncogene homolog B1

NCBI Organism

Homo sapiens

Background Reference 01

Stephens R.M., Mol. Cell. Biol. 12:3733-3742 (1992) .

Background Reference 02

Albert S., Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.

Background Reference 03

Scherer S.W., Science 300:767-772 (2003)

Other Product Names

B-RAF, RAFB1, RMIL, p94

Tested Applications

WB

Protein ID

50403720

Physical Properties

Liquid

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