P53 (Ab 6) Antibody

Product Specifications

Background

P53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes

NCBI Gene ID

7157

Swiss Prot

P04637

Accession Number

NP_000537.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

P53 (Ab-6) antibody was raised against a peptide sequence around aa.4~8 (P-Q-S-D-P) derived from Human p53.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

53 kDa

Specificity

This antibody detects endogenous level of total p53 protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

TP53

NCBI Official Name

Tumor protein p53

NCBI Organism

Homo sapiens

Background Reference 01

Lin T, et al. (2005) Nat Cell Biol; 7 (2) : 165-71. Vega FM, et al. (2004) Mol Cell Biol; 24 (23) : 10366-80. Li J, et al. (2004) J Biol Chem; 279 (40) : 41275-9. Wang J, et al. (2004) J Biol Chem; 279 (38) : 39584-92.